Inmunidad de células T específicas del SARS-CoV-2 ( COVID-19 y SARS) y controles no infectados

SARS-CoV-2-specific T cell immunity in cases of COVID-19 and SARS, and uninfected controls

Inmunidad de células T específicas del SARS-CoV-2 en casos de COVID-19 y SARS, y controles no infectados

• Nina Le Bert,
• Anthony T. Tan,
• Kamini Kunasegaran,
• Christine Y. L. Tham,
• Morteza Hafezi,
• Adeline Chia,
• Melissa Hui Yen Chng,
• Meiyin Lin,
• Nicole Tan,
• Martin Linster,
• Wan Ni Chia,
• Mark I-Cheng Chen,
• Lin-Fa Wang,
• Eng Eong Ooi,
• Shirin Kalimuddin,
• Paul Anantharajal Tambyah,
• Jenny Guek-Hong Low,
• Yee-Joo Tan &
• Antonio Bertoletti 

Nature (2020)Cite this article

• 2882 Altmetric

• Metrics details

Abstract

Memory T cells induced by previous pathogens can shape the susceptibility to, and clinical severity of, subsequent infections¹. Little is known about the presence of pre-existing memory T cells in humans with the potential to recognize SARS-CoV-2. Here, we first studied T cell responses to structural (nucleocapsid protein, NP) and non-structural (NSP-7 and NSP13 of ORF1) regions of SARS-CoV-2 in COVID-19 convalescents (n=36). In all of them we demonstrated the presence of CD4 and CD8 T cells recognizing multiple regions of the NP protein. We then showed that SARS-recovered patients (n=23) still possess long-lasting memory T cells reactive to SARS-NP 17 years after the 2003 outbreak, which displayed robust cross-reactivity to SARS-CoV-2 NP. Surprisingly, we also frequently detected SARS-CoV-2 specific T cells in individuals with no history of SARS, COVID-19 or contact with SARS/COVID-19 patients (n=37). SARS-CoV-2 T cells in uninfected donors exhibited a different pattern of immunodominance, frequently targeting the ORF-1-coded proteins NSP7 and 13 as well as the NP structural protein. Epitope characterization of NSP7-specific T cells showed recognition of protein fragments with low homology to “common cold” human coronaviruses but conserved amongst animal betacoranaviruses. Thus, infection with betacoronaviruses induces multispecific and long-lasting T cell immunity to the structural protein NP. Understanding how pre-existing NP- and ORF-1-specific T cells present in the general population impact susceptibility and pathogenesis of SARS-CoV-2 infection is of paramount importance for the management of the current COVID-19 pandemic.

Download PDF

Author information

Author notes

1. These authors contributed equally: Nina Le Bert, Anthony T. Tan

Affiliations

1. Emerging Infectious Diseases Program, Duke-NUS Medical School, Singapore, Singapore

   Nina Le Bert, Anthony T. Tan, Kamini Kunasegaran, Christine Y. L. Tham, Morteza Hafezi, Adeline Chia, Melissa Hui Yen Chng, Meiyin Lin, Nicole Tan, Martin Linster, Wan Ni Chia, Lin-Fa Wang, Eng Eong Ooi, Jenny Guek-Hong Low & Antonio Bertoletti

2. Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore, Singapore

   Meiyin Lin & Yee-Joo Tan

3. National Center of Infectious Diseases, Singapore, Singapore

   Mark I-Cheng Chen

4. Department of Infectious Diseases, Singapore General Hospital, Singapore, Singapore

   Shirin Kalimuddin & Jenny Guek-Hong Low

5. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

   Paul Anantharajal Tambyah

6. Division of Infectious Disease, University Medicine Cluster, National University Hospital, Singapore, Singapore

   Paul Anantharajal Tambyah

7. Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

   Yee-Joo Tan

8. Singapore Immunology Network, A*STAR, Singapore, Singapore

   Antonio Bertoletti

Corresponding author

Correspondence to Antonio Bertoletti.

Tenemos mucha más inmunidad de la que nos pensamos sólo que no asociada a anticuerpos sino a los linfocitos T de memoria, que no detecta un test de IgG e IgM.

Y dura mucho: gente que se infectó con el SARS-CoV hace 17 años conserva aún linfocitos-T que reconocen al SARS-CoV-2. Pero no sólo, otros coronavirus de los que nos infectamos en el pasado podrían también habernos inmunizado.

https://www.nature.com/articles/s41586-020-2550-z?fbclid=IwAR0Gw2RVEslAanwP7gePL1pY58hDezZs081mABPOpsPHPESR2jOeh347AWo